The steps for doing a restriction mapping exercise are all on this screen, so scroll down to see all of them...

1. First, use the 'Map' menu to convert the DNA fragments to mapping fragments...

...and 'mapping fragments' appear ( moveable black bars with lengths corresponding to the number of base pairs in each fragment).

2. Since some of the black bars overlap, use the 'Map' menu again and select 'spread out overlapping fragments'...

...and the individual mapping fragments are now separated, so that they can be more easily moved by dragging.

3. In the graphic below, mapping fragments have been dragged in each lane to solve the restriction map. Note from the gel label that the double-digestion is in the first lane, and the two single digestions are in lanes two and three. Similar mapping exercises can be preformed on other DNA sequences, with other enzymes.

9. Click here to go back to the "other features" menu, or else click the "Next" button to see the next feature...